Characterization of a prokaryotic SMC protein involved in chromosome partitioning
AUTOR(ES)
Britton, Robert A.
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
smc of Bacillus subtilis encodes a homolog of eukaryotic SMC proteins involved in chromosome condensation, pairing, and partitioning. A null mutation in B. subtilis smc caused a temperature-sensitive–lethal phenotype in rich medium. Under permissive conditions, the mutant had abnormal nucleoids, ∼10% of the cells were anucleate, and assembly of foci of the chromosome partitioning protein Spo0J was altered. In combination with a null mutation in spo0J, the smc mutation caused a synthetic phenotype; cell growth was slower and ∼25% of the cells were anucleate. Our results demonstrate that the B. subtilis Smc protein, like its eukaryotic counterpart, plays an important role in chromosome structure and partitioning.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=316777Documentos Relacionados
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