Characterization of bradyzoite-specific antigens of Toxoplasma gondii.
AUTOR(ES)
Tomavo, S
RESUMO
Monoclonal antibodies that react specifically with bradyzoite antigens of Toxoplasma gondii were selected by differential immunofluorescence among hybridomas produced against these organisms. These antigens were further characterized by immunofluorescence on living bradyzoites and Western immunoblotting. Four pellicular antigens (36, 34, 21, and 18 kDa) were identified; three of these are exposed on the surface of the organism and accessible to either antibodies, trypsin cleavage, or both of these surface-probing procedures. These antigens were found on recent human isolates of T. gondii, as well as on laboratory strain bradyzoites obtained from either brain cysts or in vitro-grown parasites.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=258946Documentos Relacionados
- Induction of bradyzoite-specific Toxoplasma gondii antigens in gamma interferon-treated mouse macrophages.
- Reduced replication of Toxoplasma gondii is necessary for induction of bradyzoite-specific antigens: a possible role for nitric oxide in triggering stage conversion.
- Strain-specific antigens of Toxoplasma gondii.
- Identification of stage-specific antigens of Toxoplasma gondii.
- Recognition of tachyzoite and bradyzoite antigens of Toxoplasma gondii by infected hosts.