Characterization of Clostridia by Gas Chromatography: I. Differentiation of Species by Cellular Fatty Acids
AUTOR(ES)
Moss, C. Wayne
RESUMO
Fatty acids of 41 strains representing 13 species of Clostridium were extracted directly from whole cells and examined as methyl esters by gas-liquid chromatography. Both visual and quantitative comparisons of the resulting chromatograms for the presence and relative amounts of large major peaks allowed rapid differentiation of C. perfringens, C. sporogenes, and C. bifermentans from each other and from 10 other species. Each of the three former species possessed a different characteristic fatty acid methyl ester profile that was exhibited by all strains tested within the respective species. Culture age and growth media influenced the relative proportions of certain of the acids, but such differences did not limit species differentiation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=546910Documentos Relacionados
- Characterization of Clostridia by Gas Chromatography: Differentiation of Species by Trimethylsilyl Derivatives of Whole-Cell Hydrolysates
- Differentiation of Peptococcus and Peptostreptococcus by gas-liquid chromatography of cellular fatty acids and metabolic products.
- Analysis of mycolic acid cleavage products and cellular fatty acids of Mycobacterium species by capillary gas chromatography.
- Analysis of fatty acids of the genus Rochalimaea by electron capture gas chromatography: detection of nonanoic acid.
- Discriminant analysis of cellular fatty acids of Candida species, Torulopsis glabrata, and Cryptococcus neoformans determined by gas-liquid chromatography.