Characterization of smooth lipopolysaccharides and O polysaccharides of Brucella species by competition binding assays with monoclonal antibodies.
AUTOR(ES)
Weynants, V
RESUMO
Previously, four epitope specificities on the O chain of Brucella species were reported: M, A, C, and C/Y. In this work, according to monoclonal antibody binding to smooth lipopolysaccharides of Yersinia enterocolitica 0:9, Brucella abortus W99 (A-dominant strain), and B. melitensis Rev1 (M-dominant strain), seven O-chain epitope specificities were defined: M, A, C (M > A), C (M = A), C/Y (M > A), C/Y (M = A) and C/Y (A > M). Competitive binding assays between these monoclonal antibodies suggested that these different epitopes are probably overlapping structures.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=175246Documentos Relacionados
- Immunochemical characterization of Brucella lipopolysaccharides and polysaccharides.
- Analysis of Brucella lipopolysaccharide with specific and cross-reacting monoclonal antibodies.
- Serological confirmation of Brucella abortus and Yersinia enterocolitica O:9 O-antigens by monoclonal antibodies.
- Protection against Brucella abortus in mice with O-polysaccharide-specific monoclonal antibodies.
- Rapid identification of smooth Brucella species with a monoclonal antibody.