Characterization of the auxin-inducible SAUR-AC1 gene for use as a molecular genetic tool in Arabidopsis.

AUTOR(ES)

Gil, P

RESUMO

The small auxin up RNA (SAUR) genes were originally characterized in soybean, where they encode a set of unstable transcripts that are rapidly induced by auxin. In this report, the isolation of a SAUR gene, designated SAUR-AC1, from Arabidopsis thaliana (L.) Heynh. ecotype Columbia is described. The promoter of the SAUR-AC1 gene contains putative regulatory motifs conserved among soybean SAUR promoters, as well as sequences implicated in the regulation of other genes in response to auxin. The transcribed region is approximately 500 bp in length and contains no introns. Highly conserved sequences located within the SAUR-AC1 transcript include the central portion of the coding region and a putative mRNA instability sequence (DST) located in the 3' untranslated region. Accumulation of SAUR-AC1 mRNA is readily induced by natural and synthetic auxins and by the translational inhibitor cycloheximide. Moreover, several auxin- and gravity-response mutants of Arabidopsis exhibit decreased accumulation of the SAUR-AC1 mRNA in elongating etiolated seedlings. In particular, in the axr2-1 mutant the SAUR-AC1 transcript accumulates to less than 5% of wild-type levels. These studies indicate that SAUR-AC1 will be a useful probe of auxin-induced gene expression in Arabidopsis and will facilitate the functional analysis of both transcriptional and posttranscriptional regulatory elements.

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