Characterization of the dispersin protein in Escherichia coli strains that do not belong to the enteroagreggative E.coli pathotype. / Caracterização da proteína dispersina em amostras de Escherichia coli não pertencentes ao patótipo de E. coli enteroagregativa.

AUTOR(ES)

Bianca Tomé Monteiro

DATA DE PUBLICAÇÃO

2008

RESUMO

The protein dispersin, encoded by aap, is one of the virulence factors of enteroaggregative Escherichia coli (EAEC). Detection of aap has been employed along with aggR and aatA in the molecular diagnosis of EAEC. In order to verify their specificity, these genes were searched in a collection of 243 diarrheagenic E. coli (DEC) strains. All of them were negative for aggR and aatA, whereas 26 were positive for aap. These strains have been described as atypical enteropathogenic E. coli. However, this classification was not confirmed since they did not harbor the eae gene. Three strains showed the aggregative adherence pattern to HEp-2 cells and were classified as EAEC. The remaining strains were part of a group that did not harbor any specific markers of DEC pathotypes. The DNA sequence analysis of 6 aap-harboring strains showed high homology between the sequence of these strains and the aap sequence of the EAEC prototype strain 042. This study revealed that aap is not exclusive of EAEC.

ASSUNTO(S)

diarrhea virulência escherichia coli virulence diarréia escherichia coli

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