Characterization of the Kilham Rat Virus
AUTOR(ES)
Salzman, Lois Ann
RESUMO
Kilham rat virus (KRV) was found to grow in a rat nephroma cell line and to form plaques on secondary rat embryo monolayers. The virus was purified by enzymatic treatment and isopycnic cesium chloride sedimentation. KRV bands at a density of 1.41 g/cm3 in cesium chloride. It contains about 26.5% deoxyribonucleic acid (DNA). The sedimentation coefficient S20,w in sucrose gradients was 122 corresponding to a molecular weight of 6.6 × 106 daltons. The reaction of formaldehyde with the KRV virion suggests that the DNA in situ is single-stranded. DNA extracted from KRV had a buoyant density of 1.715 g/cm3 in cesium chloride. The S20,w was determined in sucrose gradients to be 16, and the molecular weight was calculated to be approximately 1.7 × 106 daltons. The base composition of the DNA is 26.7% adenine, 30.8% thymine, 20.0% guanine, and 22.5% cytosine. On the basis of its noncomplementary nucleotide ratio, melting curve, and the reaction with formaldehyde, the DNA of KRV is believed to be single-stranded.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=375977Documentos Relacionados
- Purification of the Kilham Rat Virus
- Sequence homology between the structural proteins of Kilham rat virus.
- Host Influence on the Antigenic Composition of the Kilham Rat Virus
- Persistent Infection of a Rat Nephroma Cell Line with Kilham Rat Virus
- Isolation and structural characterization of monomeric and dimeric forms of replicative intermediates of Kilham rat virus DNA.
