Characterization of Tn5-Induced Mutants of Xenorhabdus nematophilus ATCC 19061
AUTOR(ES)
Xu, Jimin
RESUMO
A negative-selection vector, pHX1, was constructed for use in transposon mutagenesis of Xenorhabdus nematophilus ATCC 19061. pHX1 contains the Bacillus subtilis levansucrase gene which confers sucrose sensitivity. In addition, various Tn5-containing plasmids with different replication origins were transferred by conjugation from Escherichia coli into X. nematophilus ATCC 19061, and one of these plasmids, pGS9, yields Tn5 insertion mutants of X. nematophilus ATCC 19061. By using these two delivery vehicles, more than 250 putative Tn5 insertion mutants of X. nematophilus ATCC 19061 were isolated and were then characterized. Mutants that were altered in bromothymol blue adsorption, ability to lyse sheep erythrocytes, production of antibiotics on a variety of media, and virulence for Galleria mellonella were found.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=182864Documentos Relacionados
- Molecular characterization of Tn5-induced symbiotic (Fix-) mutants of Rhizobium meliloti.
- Isolation and characterization of transposon Tn5-induced symbiotic mutants of Rhizobium loti.
- Isolation and characterization of transposon Tn5-induced mutants of Pseudomonas perfectomarina defective in nitrous oxide respiration.
- Transposon Tn5-induced mutagenesis of Rhizobium japonicum yielding a wide variety of mutants.
- Cloning, characterization, and complementation of lesions causing acid sensitivity in Tn5-induced mutants of Rhizobium meliloti WSM419.