Chemical and structural alterations at the cell surface of Candida tropicalis, induced by hydrocarbon substrate.
AUTOR(ES)
Käppeli, O
RESUMO
The surface-localized polysaccharide of alkane-grown cells of Candida tropicalis was identified as mannan containing approximately 4% covalently linked fatty acids. Glucose-grown cells lacked the mannan-fatty acid complex. The surface structure of alkane-grown cells showed a radial arrangement of the wall polymers, with protruding parts. The cell surface of glucose-grown cells was smooth, with a coherent outer limit. The mannan was localized by using concanavalin A. Masking of the mannan with concanavalin A reduced the binding affinity of the surface for alkane, indicating the involvement of the surface-localized mannan-fatty acid complex in the binding of alkanes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=222108Documentos Relacionados
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