Chemical stability of bacteriophage T7 early mRNA.

AUTOR(ES)

Yamada, Y

RESUMO

T7 early mRNA produced by a gene 1 amber mutant phage, T7 am27, is chemically stable interms of acid insolubility and T7 DNA hybridizability. However, the messenger activity of individual T7 early mRNA species, transcripts of gene 1, gene 0.7, and gene 1.3, decay with a half-life of about 6.5 min at 30 C. An extensive secondary structure is present in all T7 early mRNA species and is probably responsible for the chemical stability of the RNAs after the loss of functional activity. It is unlikely that ribosomes protect T7 early mRNA from nucleolytic degradation.

Documentos Relacionados

• Effect of RNase III on the size of bacteriophage T7 lysozyme mRNA.
• Accurate in vitro cleavage by RNase III of phosphorothioate-substituted RNA processing signals in bacteriophage T7 early mRNA.
• Effect of RNase III on efficiency of translation of bacteriophage T7 lysozyme mRNA.
• Transcribing of Escherichia coli genes with mutant T7 RNA polymerases: stability of lacZ mRNA inversely correlates with polymerase speed.
• Gene-specific mRNA. II. Regulation of mRNA synthesis in E. coli after infection with bacteriophage T4.