Chemiluminescent universal probe for bacterial ribotyping.
AUTOR(ES)
Gustaferro, C A
RESUMO
We describe a novel procedure for direct covalent coupling of horseradish peroxidase to rRNA and ribotyping by using enhanced chemiluminescence. Compared with their 32P-end-labeled counterparts, chemiluminescent rRNA probes are stable and easy to synthesize and provide results as good as or superior to those obtained with isotopic labeling. Direct chemiluminescent labeling of Escherichia coli rRNA produces a sensitive, universal probe suitable for clinical laboratory use in the investigation of nosocomial outbreaks.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265214Documentos Relacionados
- Differentiation of Lactobacillus strains by ribotyping.
- Assessment of a chemiluminescent universal probe for taxonomical and epidemiological investigations of Aeromonas sp isolates.
- Epidemiological differentiation of pathogenic strains of Salmonella enteritidis by ribotyping.
- Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping.
- Molecular analysis of Salmonella enteritidis by pulsed-field gel electrophoresis and ribotyping.