Chemiosmotic Energy Conservation with Na+ as the Coupling Ion during Hydrogen-Dependent Caffeate Reduction by Acetobacterium woodii
AUTOR(ES)
Imkamp, Frank
FONTE
American Society for Microbiology
RESUMO
Cell suspensions of Acetobacterium woodii prepared from cultures grown on fructose plus caffeate catalyzed caffeate reduction with electrons derived from molecular hydrogen. Hydrogen-dependent caffeate reduction was strictly Na+ dependent with a Km for Na+ of 0.38 mM; Li+ could substitute for Na+. The sodium ionophore ETH2120, but not protonophores, stimulated hydrogen-dependent caffeate reduction by 280%, indicating that caffeate reduction is coupled to the buildup of a membrane potential generated by primary Na+ extrusion. Caffeate reduction was coupled to the synthesis of ATP, and again, ATP synthesis coupled to hydrogen-dependent caffeate reduction was strictly Na+ dependent and abolished by ETH2120, but not by protonophores, indicating the involvement of a transmembrane Na+ gradient in ATP synthesis. The ATPase inhibitor N,N′-dicyclohexylcarbodiimide (DCCD) abolished ATP synthesis, and at the same time, hydrogen-dependent caffeate reduction was inhibited. This inhibition could be relieved by ETH2120. These experiments are fully compatible with a chemiosmotic mechanism of ATP synthesis with Na+ as the coupling ion during hydrogen-dependent caffeate reduction by A. woodii.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=134933Documentos Relacionados
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