Chloroplast Response to Low Leaf Water Potentials: IV. Quantum Yield Is Reduced 1

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Quantum yields were measured for CO2 fixation by sunflower (Helianthus annuus L.) leaves having various water potentials and for dichlorophenolindophenol photoreduction by chloroplasts isolated from similar leaves having various water potentials. In red radiation, the quantum yield for CO2 was 0.076 for an attached sunflower leaf at a water potential of −3 to −4 bars but was 0.020 for the same leaf at −15.3 bars. After recovery to a water potential of −5 bars, the quantum yield rose to 0.060. Soybean (Glycine max L. [Merr.]) leaves behaved similarly. Chloroplasts from a sunflower leaf with a water potential of −3.6 bars had a quantum yield for 4 equivalents of 0.079, but when tissue from the same leaf had a water potential of −14.8 bars, the quantum yield of the chloroplasts decreased to 0.028. The decrease could not be attributed to differences in rates of respiration by the leaves or the chlorophyll content or absorption spectrum of the leaves and chloroplasts.

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