Chromosomal gene capture mediated by the Pseudomonas putida TOL catabolic plasmid.
AUTOR(ES)
Ramos-González, M I
RESUMO
The Pseudomonas putida TOL plasmid pWW0 is able to mediate chromosomal mobilization in the canonical unidirectional way, i.e., from donor to recipient cells, and bidirectionally, i.e., donor-->recipient-->donor (retrotransfer). Transconjugants are recipient cells that have received DNA from donor cells, whereas retrotransconjugants are donor bacteria that have received DNA from a recipient. The TOL plasmid pWW0 is able to directly mobilize and retromobilize a kanamycin resistance marker integrated into the chromosome of other P. putida strains, a process that appears to involve a single conjugational event. The rate of retrotransfer (as well as of direct transfer) of the chromosomal marker is influenced by the location of the kanamycin marker on the chromosome and ranges from 10(-3) to less than 10(-8) retrotransconjugants per donor (transconjugants per recipient). The mobilized DNA is incorporated into the chromosome of the retrotransconjugants (transconjugants) in a process that seems to occur through recombination of highly homologous flanking regions. No interspecific mobilization of the chromosomal marker in matings involving P. putida and the closely related Pseudomonas fluorescens, which belongs to rRNA group I, was observed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=196284Documentos Relacionados
- The TOL (pWW0) catabolic plasmid.
- Chromosomal location of TOL plasmid DNA in Pseudomonas putida.
- Carbon source-dependent inhibition of xyl operon expression of the Pseudomonas putida TOL plasmid.
- Growth-phase-dependent expression of the Pseudomonas putida TOL plasmid pWW0 catabolic genes.
- Involvement of IHF protein in expression of the Ps promoter of the Pseudomonas putida TOL plasmid.