Chromosomal location of a Bacillus subtilis DNA fragment uniquely transcribed by sigma-28-containing RNA polymerase.
AUTOR(ES)
Ferrari, F A
RESUMO
A fragment of the Bacillus subtilis chromosome containing a gene whose transcription is dependent on sigma-28-containing RNA polymerase has been genetically mapped by means of an integrable plasmid. This gene resides on the chromosome adjacent to the stage 0 sporulation locus spo0E between metC and citL. The gene was insertionally inactivated by cloning an internal EcoRI-HindIII fragment in the integrable plasmid pJH101 and by inserting the plasmid into the chromosome by transformation. Transformants bearing an inactivated gene were indifferent to this inactivation for both growth and sporulation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=221529Documentos Relacionados
- The Bacillus subtilis flagellin gene (hag) is transcribed by the sigma 28 form of RNA polymerase.
- Nucleotide sequences of two Bacillus subtilis promoters used by Bacillus subtilis sigma-28 RNA polymerase.
- Bacillus subtilis spoVE gene is transcribed by sigma E-associated RNA polymerase.
- An operon of Bacillus subtilis motility genes transcribed by the sigma D form of RNA polymerase.
- Nucleotide sequence of a Bacillus subtilis promoter recognized by Bacillus subtilis RNA polymerase containing sigma 37.