Chromosome Transfer and Recombinant Formation with Deoxyribonucleic Acid Temperature-Sensitive Strains of Escherichia coli
AUTOR(ES)
Stallions, Donald R.
RESUMO
Haploid recombinant yield is reduced in matings conducted at 42.5 C between deoxyribonucleic acid (DNA) temperature-sensitive [dna−(TS)] recipients unable to synthesize DNA at 42.5 C and any of the three major donor types (Hfr, F+, F′) of Escherichia coli. No such reduction is observed in matings conducted at 42.5 C when the dna−(TS) mutation is in the donor parent. Evidence is presented which indicates that chromosome transfer from donors to recipients unable to replicate DNA at 42.5 C during vegetative growth occurs at normal frequencies when the mating is conducted at 42.5 C. It is concluded that some stage in haploid recombinant formation is adversely affected in dna−(TS) recipients mated at the temperature restrictive for DNA synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=248514Documentos Relacionados
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