Circular Deoxyribonucleic Acid from Shigella dysenteriae Y6R

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Circular deoxyribonucleic acid was isolated from Shigella dysenteriae Y6R and was found to consist of six species having molecular weights of 106, 1.3 × 106, 2.6 × 106, 3.8 × 106, 20 × 106, and 24 × 106 daltons. These size classes were partially resolved by sucrose density gradient centrifugation. The minicircles (106 and 1.3 × 106) were found to have a buoyant density in CsCl of 1.710 g/ml. The 3.8 × 106 dalton class had a density of 1.707 g/ml. The two largest species had a density of 1.702 g/ml. Two other strains, S. sonnei II and S. dysenteriae 60, also contained circular deoxyribonucleic acid.

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