cis-Acting Sequences Required for Simian Foamy Virus Type 1 Vectors
AUTOR(ES)
Wu, Min
FONTE
American Society for Microbiology
RESUMO
We have constructed a series of vectors based on simian foamy virus type 1 (SFV-1) to define the minimum cis-acting elements required for gene transfer. To characterize these vectors, we inserted the coding sequence of the bacterial lacZ gene linked to the cytomegalovirus immediate-early gene promoter. Introduction of a deletion mutation in the leader region between the 5′ long terminal repeat and the start of the gag gene at position 1659 to 1694 completely abrogated gene transfer by the SFV-1 vector. Deletion of 39 nucleotides from position 1692 to 1731 in the leader region resulted in a significant reduction in the transducing-particle titer. Furthermore, we have identified a second cis-acting element located at the 3′ end of the pol gene between position 6486 and 6975 to be critical for SFV-1 vector transduction. These results identify the two important cis-acting elements required for SFV-1 vector construction, and the finding of a cis-acting element in the pol gene is unique among retroviruses.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=109851Documentos Relacionados
- cis-acting regulatory regions in the long terminal repeat of simian foamy virus type 1.
- Characterization of a cis-Acting Sequence in the pol Region Required To Transfer Human Foamy Virus Vectors
- cis-acting sequences involved in human immunodeficiency virus type 1 RNA packaging.
- cis-acting sequences required for encapsidation of duck hepatitis B virus pregenomic RNA.
- Delineation of Cis-Acting Sequences Required for Expression of Drosophila Mojavensis Adh-1
