ClaI. a new restriction endonuclease from Caryophanon latum L.
AUTOR(ES)
Mayer, H
RESUMO
From Caryophanon latum L site specific restriction endonuclease (ClaI) has been purified, which recognises tha DNA hexanucleotide palindrome 5'-A-T-C-G-A-T-3'. Staggered cleavage generates DNA restriction fragments with 5'-terminal pCG extensions. A CLaI map of bacteriophage lambda has been determined, which indicates cleavage inhibition due to adenine methylation at over lapping ClaI-GATC recognition sequences. Plasmid pBR322 is cut only once, in the tetracycline promoter region, and can, therefore, be used as a vector system for cloning fragments derived from ClaI digestions, and in addition for fragments generated by TaqI, HpaII, and several other enzymes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=327483Documentos Relacionados
- Purification and characterization of two new modification methylases: MClaI from Caryophanon latum L and MTaqI from Thermus aquaticus YTI.
- Isolamento e caracterização de BliAI, um isoesquisômero de ClaI de Bacillus licheniformis
- RESPONSE OF CARYOPHANON LATUM TO OXYGEN
- Isolation of Bacteriophage for Caryophanon latum
- Defined Liquid Minimal Medium for Caryophanon latum
