Cloned pairs of variable region genes for immunoglobulin heavy chains isolated from a clone library of the entire mouse genome.
AUTOR(ES)
Kemp, D J
RESUMO
To investigate the organization of immunoglobulin genes, we have constructed a clone library containing 10(6) randomly generated fragments of mouse embryo DNA, corresponding to eight equivalents of the genome. The cloning method involved methylation of embryo DNA at EcoRI recognition sites, partial digestion by EcoRI* endonclease activity, and direct ligation of the resulting large fragments to the lambda phage vector Charon 4A. The library was searched for sequences homologous to a cloned complementary DNA copy of a mu heavy chain mRNA. Nine clones bearing variable heavy chain (VH) sequences were isolated, representing at least eight distinct VH genes. Thus, multiple related VH genes are available in the genome to contribute to immunoglobulin diversity. Each of the two clones carries a pair of VH genes, one pair separated by 15 +/- 1 kilobase pairs of mouse DNA and the other by 14 +/- 2 kilobase pairs. This indicates that related VH genes are clustered and may occur in a tandem array having a repeating unit of 14--16 kilobase pairs. The large spacer sequences between VH genes cannot, however, be highly conserved.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411632Documentos Relacionados
- Mouse Immunoglobulin Heavy Chains Are Coded by Multiple Germ Line Variable Region Genes
- Structure and multiplicity of genes for the human immunoglobulin heavy chain variable region.
- Structure of immunoglobulin gamma 2b heavy chain gene cloned from mouse embryo gene library.
- Characterization of a mouse DNA clone containing an immunoglobulin variable region gene.
- Mouse immunoglobulin genes: a bacterial plasmid containing the entire coding sequence for a pre-gamma 2a heavy chain.