Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans.
AUTOR(ES)
Yasui, A
RESUMO
A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacZ of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E. coli photolyases, notwithstanding the difference in chromophore structure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=336641Documentos Relacionados
- Cloning of nitrate reductase genes from the cyanobacterium Anacystis nidulans.
- Shuttle cloning vectors for the cyanobacterium Anacystis nidulans.
- Sequence of the ribonuclease P RNA gene from the cyanobacterium Anacystis nidulans.
- Regulated nitrate transport in the cyanobacterium Anacystis nidulans.
- Regulatory properties of a fructose 1,6-bisphosphatase from the cyanobacterium Anacystis nidulans.