Cloning and characterization of a sixth adenylyl cyclase isoform: types V and VI constitute a subgroup within the mammalian adenylyl cyclase family.

AUTOR(ES)

Katsushika, S

RESUMO

A sixth member of the mammalian adenylyl cyclase family has been isolated from a canine cardiac cDNA library. This isoform is more highly homologous to type V than to the other adenylyl cyclase types; sequence similarity is apparent even in the transmembrane regions where the greatest divergence among the types exists. Type VI mRNA expression is most abundant in heart and brain; however, unlike type V, a low level of expression is also observed in a variety of other tissues examined. Type VI adenylyl cyclase can be stimulated by NaF, guanosine 5'-[gamma-thio]triphosphate, and forskolin but not by Ca2+/calmodulin, whereas it is inhibited by adenosine and its analogues. Comparison of both their structural and biochemical properties suggests that types V and VI constitute a distinct subgroup of the mammalian adenylyl cyclase family.

Documentos Relacionados

• Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
• Cloning and characterization of myr 6, an unconventional myosin of the dilute/myosin-V family.
• The Dictyostelium homologue of mammalian soluble adenylyl cyclase encodes a guanylyl cyclase
• Cloning and characterization of a second member of the mouse mdr gene family.
• Molecular cloning and characterization of a Ca2+/calmodulin-insensitive adenylyl cyclase from rat brain.