Cloning and characterization of the beta-amylase gene from Bacillus polymyxa.
AUTOR(ES)
Friedberg, F
RESUMO
The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydrolysis with this beta-amylase produces maltose, not glucose, whereas maltotriose and cycloheptaose are resistant to the action of this beta-amylase. The enzyme has a molecular weight of approximately 68,000. Restriction endonuclease mapping demonstrated that the DNA inserted in pBD64 and containing the gene is approximately 3 kilobases in length.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=214501Documentos Relacionados
- Cloning of the beta-amylase gene from Bacillus cereus and characteristics of the primary structure of the enzyme.
- Beta-Amylase Production by Bacillus polymyxa on a Corn Steep-Starch-Salts Medium
- Cloning and sequencing of the gene encoding thermophilic beta-amylase of Clostridium thermosulfurogenes.
- Cloning and nucleotide sequence of the gene coding for enzymatically active fragments of the Bacillus polymyxa beta-amylase.
- Proteases involved in generation of beta- and alpha-amylases from a large amylase precursor in Bacillus polymyxa.