Cloning and Detection of Chromosomal and Extrachromosomal DNA from Mycoplasmalike Organisms That Cause Yellow Dwarf Disease of Rice
AUTOR(ES)
Nakashima, Kazuo
RESUMO
DNA was extracted from rice plants infected with mycoplasmalike organisms (MLOs) causing yellow dwarf disease. DNA of the causal agent was separated from the host DNA by repeated bisbenzimide-CsCl equilibrium density gradient centrifugations. MLO DNA cut by HindIII was ligated into plasmid Bluescript II and cloned in Escherichia coli NM522. The DNA inserts were labeled with peroxidase and employed as probes in hybridization. Southern analysis revealed that the insert in pRYD-12 consisted of one, presumably chromosomal, piece of MLO DNA, whereas the insert in pRYD-19, another recombinant plasmid, consisted of one, presumably extrachromosomal, piece of MLO DNA. Cloned DNA probes were successfully applied in dot blot hybridization for the detection of rice yellow dwarf disease MLOs in rice plants and in an insect vector, the green rice leafhopper (Nephotettix cincticeps).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=184014Documentos Relacionados
- Identification and analysis of a genomic strain cluster of mycoplasmalike organisms associated with Canadian peach (eastern) X disease, western X disease, and clover yellow edge.
- Mycoplasmalike organisms (MLOs) and distinguish them from other MLOs.
- 16S rRNA sequence indicates that plant-pathogenic mycoplasmalike organisms are evolutionarily distinct from animal mycoplasmas.
- Cloning and sequence analysis of a cDNA encoding the capsid protein of the MAV isolate of barley yellow dwarf virus.
- Tandem Repeats in Extrachromosomal Ribosomal DNA of Dictyostelium Discoideum, Resulting from Chromosomal Mutations