Cloning and expression of bacterial ice nucleation genes in Escherichia coli.
AUTOR(ES)
Orser, C
RESUMO
Epiphytic populations of Pseudomonas syringae and Erwinia herbicola are important sources of ice nuclei that incite frost damage in agricultural crop plants. We have cloned and characterized DNA segments carrying the genes (ice) responsible for the ice-nucleating ability of these bacteria. The ice region spanned 3.5 to 4.0 kilobases and was continuous over this region in P. syringae Cit7R1. The cloned fragments imparted ice-nucleating activity in Escherichia coli. Substantial increases in the nucleating activity of both E. coli and P. syringae were obtained by subcloning the DNA fragments on multicopy plasmid vectors. Southern blot analysis showed substantial homology between the ice regions of P. syringae and E. herbicola, although individual restriction sites within the ice regions differed between the two species.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=214252Documentos Relacionados
- Cloning and expression of Treponema pallidum (Nichols) antigen genes in Escherichia coli.
- Cloning and expression of Thiobacillus ferrooxidans mercury ion resistance genes in Escherichia coli.
- Cloning and expression of Bradyrhizobium japonicum uptake hydrogenase structural genes in Escherichia coli.
- Cloning of Beneckea genes in Escherichia coli.
- Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli.