Cloning and Expression of clt Genes Encoding Milk-Clotting Proteases from Myxococcus xanthus 422
AUTOR(ES)
Poza, M.
FONTE
American Society for Microbiology
RESUMO
The screening of a gene library of the milk-clotting strain Myxococcus xanthus 422 constructed in Escherichia coli allowed the description of eight positive clones containing 26 open reading frames. Only three of them (cltA, cltB, and cltC) encoded proteins that exhibited intracellular milk-clotting ability in E. coli, Saccharomyces cerevisiae, and Pichia pastoris expression systems.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=522063Documentos Relacionados
- Production of an extracellular milk-clotting activity during development in Myxococcus xanthus.
- Milk-Clotting Enzymes From Microorganisms
- Potential of quixaba (Sideroxylon obtusifolium) latex as a milk-clotting agent
- Comparative evaluation of Bacillus licheniformis 5A5 and Aloe variegata milk-clotting enzymes
- Jacaratia corumbensis O. Kuntze a new vegetable source for milk-clotting enzymes