Cloning and sequencing of a rearranged V lambda gene from a Burkitt's lymphoma cell line expressing kappa light chains.
AUTOR(ES)
Sun, L H
RESUMO
We have cloned and sequenced a rearranged V lambda gene from a Burkitt's lymphoma cell line PA682(PB). This cell line has two rearranged kappa loci and has been shown to be expressing kappa light chains. This V lambda gene has been identified as a member of the V lambda subgroup III gene family based on the homology of the predicted amino acid sequence of PAV lambda with the reported sequences of the V lambda protein DEL of subgroup III. Nine cross-hybridizing bands have been detected on Southern blots and the chromosomal orientation of the V lambda subgroup III gene family has been determined in relation to the V lambda subgroup I gene family. Although the PAV lambda rearrangement has occurred via a legitimate V-J joining and a normal size transcript is detected on Northern blots, the nucleotide sequence reveals a high level of mutations resulting in multiple termination signals within the V gene coding sequence and only a truncated V lambda protein can be translated. This confirms previous observations that although multiple light chain genes may be transcribed, only one functional light chain protein can be synthesized.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321835Documentos Relacionados
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