Cloning of heat-shock locus 93D from Drosophila melanogaster.
AUTOR(ES)
Walldorf, U
RESUMO
Using the microcloning approach a number of recombinant lambda phages carrying DNA from the 93D region have been isolated. Screening genomic libraries, cloned in phage lambda or cosmid vectors, with this isolated DNA yielded a series of overlapping DNA fragments from the region 93D6-7 as shown by in situ hybridization to polytene chromosomes. In vitro 32P-labelled nuclear RNA prepared from heat-shocked third instar larvae hybridized specifically to one fragment within 85 kb of cloned DNA. The region which is specifically transcribed after heat shock could be defined to a cluster of internally-repetitive DNA and its neighbouring proximal sequences. Over a sequence of 10-12 kb in length the DNA is cut into repeat units of approximately 280 nucleotides by the restriction endonuclease TaqI. The TaqI repeat sequences are unique in the Drosophila genome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=557719Documentos Relacionados
- Mutational Analysis of the Region Surrounding the 93d Heat Shock Locus of DROSOPHILA MELANOGASTER
- Heat shock locus 93D of Drosophila melanogaster: a spliced RNA most strongly conserved in the intron sequence.
- Heat-shock puff 93 D from Drosophila melanogaster: accumulation of a RNP-specific antigen associated with giant particles of possible storage function.
- Organization of the multiple genes for the 70,000-dalton heat-shock protein in Drosophila melanogaster.
- Heat shock loci 93D of Drosophila melanogaster and 48B of Drosophila hydei exhibit a common structural and transcriptional pattern.