Cloning of high molecular weight gluten subunit promoter and study on its function in wheat

AUTOR(ES)

Weibo, Jin, Jin, Liu, Fangli, Wu, Aiguang, Guo

FONTE

Brazilian Archives of Biology and Technology

DATA DE PUBLICAÇÃO

2009-04

RESUMO

The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds.

Documentos Relacionados

• Impact of high-molecular-weight glutenin alleles on wheat technological quality
• Function and Immunochemistry of Prekallikrein-High Molecular Weight Kininogen Complex in Plasma
• Heterologous expression of a wheat high molecular weight glutenin gene in Escherichia coli.
• Tissue-specific expression of a wheat high molecular weight glutenin gene in transgenic tobacco.
• Methylation of high-molecular-weight subunit RNA of feline leukemia virus.