Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.
AUTOR(ES)
Colbere-Garapin, F
RESUMO
A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned in Escherichia coli. A recombinat plasmid, pFG5, has been analyzed extensively and a detailed restriction map is presented. pFG5 DNA efficiently transforms TK- mouse L cells. The TK coding sequence in the cloned fragment has been localized and a smaller recombinant plasmid, pAG0, also carrying an active TK gene, has been constructed to serve as a more convenient vector for transfer, into TK- cells, of genes previously cloned in E. coli.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=383912Documentos Relacionados
- Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12
- Expression of herpes simplex virus type I thymidine kinase gene in Escherichia coli.
- Herpes simplex virus thymidine kinase activity of thymidine kinase-deficient Escherichia coli K-12 mutant transformed by hybrid plasmids.
- Location and cloning of the herpes simplex virus type 2 thymidine kinase gene.
- Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.
