Cloning of the gene, speB, for streptococcal pyrogenic exotoxin type B in Escherichia coli.
AUTOR(ES)
Bohach, G A
RESUMO
The structural gene encoding streptococcal pyrogenic exotoxin type B, designated speB, was cloned in Escherichia coli and localized onto a 4.5-kilobase BamHI-BglII DNA fragment. Streptococcal pyrogenic exotoxin type B, partially purified from E. coli clones, was immunologically related to streptococcus-derived toxin. Also, toxin derived from either E. coli or Streptococcus pyogenes had similar lymphocyte mitogenic activity and molecular weight (29,300) and displayed comparable microheterogeneity when evaluated by isoelectric focusing.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=259455Documentos Relacionados
- Locations of the speA, speB, speC, and metK genes on the physical map of Escherichia coli.
- Analysis and sequence of the speB gene encoding agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli.
- Temperature regulation of the streptococcal pyrogenic exotoxin A-encoding gene (speA).
- Inactivation of the streptococcal erythrogenic toxin B gene (speB) in Streptococcus pyogenes.
- The M Protein Is Dispensable for Maturation of Streptococcal Cysteine Protease SpeB