Cloning, sequence, and expression of bovine interleukin 2.
AUTOR(ES)
Cerretti, D P
RESUMO
Interleukin 2 (IL-2) cDNA clones have been isolated from both human and murine sources. We report here the isolation of a cDNA clone encoding bovine IL-2. This was accomplished by screening a cDNA library constructed from lectin-stimulated bovine lymph node cells, using a human IL-2 probe. Bovine IL-2 is composed of 155 amino acids and has a predicted molecular weight of 19,555. Alignment of the amino acid sequence with human IL-2 indicates that mature bovine IL-2 is composed of 135 amino acids and has a predicted molecular weight of 15,452. It has an amino acid homology of 65% with human IL-2 and 50% with murine IL-2. Bovine IL-2 is unique among IL-2 homologs in that it has a single N-linked glycosylation site. Biologically active bovine IL-2 was synthesized in an Escherichia coli expression system.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=323485Documentos Relacionados
- Molecular cloning, sequence, expression, and processing of the interleukin 16 precursor
- Cloning, DNA Sequence, and Expression of the Rhodobacter sphaeroides Cytochrome c2 Gene
- Cloning, DNA sequence, and expression of the Rhodobacter sphaeroides cytochrome c2 gene.
- Molecular cloning, nucleotide sequence, and expression of the gene encoding human eosinophil differentiation factor (interleukin 5).
- Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene.