Cloning, Sequencing, and Phenotypic Characterization of the rpoS Gene from Pseudomonas putida KT2440
AUTOR(ES)
Ramos-González, María Isabel
FONTE
American Society for Microbiology
RESUMO
A gene homologous to the rpoS gene of Escherichia coli was cloned from a Pseudomonas putida KT2440 gene bank by complementation of the rpoS-deficient strain E. coli ZK918. The rpoS gene of P. putida complemented the acid sensitivity and catalase deficiency of the rpoS mutant of E. coli and stimulated expression of the RpoS-controlled promoter, bolAp1. The gene was sequenced and found to be highly similar to the rpoS genes of other gram-negative bacteria. Like in other gram-negative bacteria, a homolog of the nlpD gene was found upstream to the rpoS gene. A transcriptional fusion of the promoter of the P. putida rpoS gene to the luxAB genes from Vibrio harveyi was constructed and used as an inactivated allele of rpoS for gene replacement of the wild-type copy in the chromosome of P. putida. The resultant rpoS mutant of P. putida, C1R1, showed reduced survival of carbon starvation and reduced cross-protection against other types of stress in cells starved for carbon, in particular after a challenge with ethanol. Survival in soil amended with m-methylbenzoate was also reduced in the mutant strain P. putida C1R1. The RpoS protein of P. putida controls the expression of more than 50 peptides, which are normally expressed in cells after a short period of carbon starvation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=107299Documentos Relacionados
- Combined Physical and Genetic Map of the Pseudomonas putida KT2440 Chromosome
- Cell Density-Dependent Gene Contributes to Efficient Seed Colonization by Pseudomonas putida KT2440
- Hydroxyectoine Is Superior to Trehalose for Anhydrobiotic Engineering of Pseudomonas putida KT2440
- Cloning, sequencing, and expression of the Pseudomonas putida protocatechuate 3,4-dioxygenase genes.
- Cloning, sequencing, and mutation of a gene for azurin in Methylobacillus flagellatum KT.