CO2-Responsive Transcriptional Regulation of CAH1 Encoding Carbonic Anhydrase Is Mediated by Enhancer and Silencer Regions in Chlamydomonas reinhardtii1
AUTOR(ES)
Kucho, Ken-ichi
FONTE
American Society of Plant Physiologists
RESUMO
Chlamydomonas reinhardtii adapts to the stress of CO2-limiting conditions through the induction of a set of genes including CAH1, which encodes a periplasmic carbonic anhydrase. CAH1 is up-regulated under low-CO2 conditions (air containing 0.04% [v/v] CO2) in the presence of light, whereas it is down-regulated under high-CO2 conditions (5% [v/v] CO2) or in the dark. In an effort to identify cis-elements involved in the transcriptional regulation of CAH1, a series of 5′-nested deletions of the region upstream of CAH1 were fused to a promoterless arylsulfatase reporter gene (ARS). The upstream region from −651 to +41 relative to the transcription start site was sufficient to regulate the expression of ARS with kinetics similar to those of endogenous CAH1. Deletion of the region between −651 and −294 resulted in a significant decrease in the level of arylsulfatase activity expressed under low-CO2 conditions. The 543-bp upstream region from −651 to −109, without any promoter elements, CAAT-box, or TATA-box, could confer CO2 and light responsiveness on the β2-tubulin minimal promoter. This 543-bp region was divided into two parts: a 358-bp silencer region from −651 to −294, which represses the minimal promoter activity under high-CO2 conditions, and a 185-bp enhancer region from −293 to −109, which activates the promoter under low-CO2 conditions in the presence of light.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=59500Documentos Relacionados
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