Colorimetric detection of immobilised PCR products generated on a solid support.
AUTOR(ES)
Lockley, A K
RESUMO
By extending functional primers attached to a solid phase and incorporating a digoxigenin label, it is possible to visualise PCR products as discrete spots on specific regions of a solid support after colorimetric detection. The technique has been used for the detection of the point mutation associated with porcine malignant hyperthermia.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=146568Documentos Relacionados
- The kinetics of phosphotriester condensation reactions on solid support.
- Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support.
- Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.
- Solid support quantitation of c-myc PCR products using a cleavable reporter.
- Primer specific solid-phase detection of PCR products.