Comparação entre teste bana e checkerboard dna-dna hybridization no diagnóstico periodontal inicial e no monitoramento terapêutico

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

The aim of this study was tom compare the results of the BANA with the presence of the red complex microorganisms established by the Checkerboard DNA-DNA hybridization in the initial diagnosis and in the therapeutic maintenance of subjects with periodontitis. 54 subjects with chronic periodontitis with at least 15 teeth were selected. Subgingival plaque samples were collected with Gracey curettes from 6 periodontal sites per subject with periodontal probing depths between 5-7mm and clinical attachment level between 5-10mm, in four experimental times: initial diagnosis (T0), immediately (T1), 45 (T2) and 60 (T3) days after scaling and root planing. The identification of Porphyromonas gingivalis, Tannerella forsythensis and/or Treponema denticola was determined by the BANA Test and by Checkerboard DNA-DNA hybridization. For both tests in T0 the highest frequencies were of positive results, and in T3 of negative results (Wilcoxon, p <0,05). In the four experimental times, a strong enzymatic activity (score 2) was accompanied by high proportions (≥105 cels) of P. gingivalis and T. forsythensis (Wilcoxon, p <0,05). There was an association between the BANA Test and the Checkerboard DNA-DNA hybridization for the "red complex", only in the initial exam (X2=10,79 and p=0,001). The sensitivity of the BANA Test for the identification of the "red complex" was as follows 84,13% (T0), 42,54% (T1), 40,76% (T2) and 28,57% (T3); and the specificity was 39,53% (T0), 67,93% (T1), 68,66% (T2) and 80,42% (T3). The BANA Test showed to be a sensitive method for the detection of the periodontal pathogens of the "red complex" in the initial diagnosis. The presence and subgingival levels of P. gingivalis, T. denticola and T. forsythensis did not show a direct association with the different levels of the enzymatic activity.

ASSUNTO(S)

periodontite diagnosis enzimas periodontitis bacteria nucleic acid hybridization hibridização de ácido nucléico diagnóstico enzymes odontologia bactérias

ACESSO AO ARTIGO

Documentos Relacionados