Comparative evaluation of several techniques for purification of Cryptosporidium parvum oocysts from rat feces.
AUTOR(ES)
Suresh, P
RESUMO
Three methods of isolating Cryptosporidium parvum oocysts from rat feces were evaluated. Oocysts were initially isolated by sucrose density gradient centrifugation. They were further purified by passage through a glass bead column or a Percoll gradient or by dialysis. Although oocysts recovered from the glass bead column and by dialysis were relatively free of fecal debris, only oocysts recovered from the Percoll gradient were free of bacteria. Recovered oocysts retained their antigenicity and infectivity. The ability to effectively recover oocysts from rat feces suggests that the laboratory rat may be a convenient substitute for ruminants in the propagation and maintenance of C. parvum oocysts for in vitro and in vivo use.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=228725Documentos Relacionados
- Effect of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine feces.
- Method for Detection and Enumeration of Cryptosporidium parvum Oocysts in Feces, Manures, and Soils
- Evaluation of Immunomagnetic Separation for Recovery of Infectious Cryptosporidium parvum Oocysts from Environmental Samples
- Evaluation of Cryptosporidium parvum Genotyping Techniques
- Characterization of the Cryptosporidium antigens from sporulated oocysts of Cryptosporidium parvum.
