Comparative study of cultivation of feces in vermiculite or charcoal to obtain larvae of Strongyloides venezuelensis
AUTOR(ES)
Ribeiro, Steveen Rios, Maia, Caroline Ohnesorge, Pereira, Fausto Edmundo Lima, Moreira, Narcisa Imaculada Brant
FONTE
Rev. Soc. Bras. Med. Trop.
DATA DE PUBLICAÇÃO
2014-10
RESUMO
IntroductionWe compared feces culturing in charcoal or vermiculite to obtain Strongyloides venezuelensis larvae.MethodsFeces (5g) from infected rats was mixed with vermiculite (10g) or coal (10g) in plastic cups and incubated at 28°C for 48h. Larvae were recovered using Baermann-Moraes method.ResultsSignificantly higher number of positive larval cultures were recovered from vermiculite than from charcoal (15/17 and 4/17, respectively; p < 0.001; 990.6 ± 307.5 and 215 ± 78.1 larvae, p = 0.027).ConclusionsVermiculite yields more larvae and provides cleaner pellets, improving larvae identification and facilitating their use for other purposes.
Documentos Relacionados
- In vitro efficacy of latex and purified papain from Carica papaya against Strongyloides venezuelensis eggs and larvae
- Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats
- Genetic basis of the resistance to Strongyloides venezuelensis (Nematoda, Rhabdiasidae) infection in mice (Mus musculus)
- Caracterização do complexo proteolitico proteassoma em Strongyloides venezuelensis
- Interferência da dexametasona no ciclo pulmonar da infecção por Strongyloides venezuelensis em ratos Wistar