Comparison between continuous and batch processing to produce xylanase by penicillium canescens 10-10c
AUTOR(ES)
Bakri, Y., Akeed, Y., Thonart, P.
FONTE
Braz. J. Chem. Eng.
DATA DE PUBLICAÇÃO
2012-09
RESUMO
Penicillium canescens 10-10c strain was cultivated on barley straw hydrolysate as a soluble nutrient source and as inducer for xylanase production. Barley straw hydrolysate was obtained by treatment of barley straw with NaOH or hot water. In shake flask cultures, NaOH treatment was found to increase the biomass production, but was not accompanied by an increase in xylanase production. The best xylanase production (54 U/ml) was observed on hydrolyzed extract from barley straw treated with hot water (100 ºC) for 3 hours. Enzyme production was further improved by scaling up the cultivation process to a 3-L stirred tank bioreactor. For batch cultivations in the bioreactor, the maximum xylanase productivity reached 1.31 and 0.46 U/ml/h, respectively, after 96 and 168 hours of cultivation. However, xylanase productivity reached 3.46 U/ml/h in the continuous culture. These results suggest that xylanase can be produced efficiently by Penicillium canescens 10-10c in continuous culture from an inexpensive source such as barley straw hydrolysate.
Documentos Relacionados
- Comparisons between continuous and batch processing to produce clavulanic acid by Streptomyces clavuligerus
- Role of Penicillic Acid in the Phytotoxicity of Penicillium cyclopium and Penicillium canescens to the Germination of Corn Seeds
- Partial purification and characterization of xylanase produced by Penicillium expansum
- Improvement of submerged culture conditions to produce colorants by Penicillium purpurogenum
- Batch and continuous extraction of bromelain enzyme by reversed micelles