Comparison between Indirect Immunofluorescence Assay and Shell Vial Culture for Detection of Mumps Virus from Clinical Samples
AUTOR(ES)
Reina, Jordi
FONTE
American Society for Microbiology
RESUMO
We report a prospective comparison of the efficacies of an indirect immunofluorescence assay (IFA) and shell vial culture (SVC) of throat swab and urine samples from patients with mumps. Throat swab samples were used for the IFA; the urine samples and throat swabs were inoculated into vials of Vero cells. We studied 62 patients by using 62 throat swabs and 50 urine samples (50 patients with both samples). Sixty (96.7%) throat samples were positive in the SVC, and 61 (98.3%) were positive in the IFA. For the 50 patients from whom both samples were available, the IFA was positive in 50 (100%) cases, the urine sample was positive in 49 (98%) cases, and the throat swab was positive in 48 (96%) cases (P > 0.05). This comparison of throat swabs and urine samples has shown that the two clinical samples are similar in efficacy.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=262465Documentos Relacionados
- Comparison of cytomegalovirus antigenemia assay with shell vial-indirect immunofluorescence assay for rapid detection of cytomegalovirus in blood.
- Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay.
- Comparison of standard culture methods, a shell vial assay, and a DNA probe for the detection of herpes simplex virus.
- Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens.
- Rapid detection of polyomavirus BK by a shell vial cell culture assay.
