Comparison of an enzyme-linked immunosorbent assay with indirect hemagglutination and hemagglutination inhibition for determination of rubella virus antibody: evaluation of immune status with commercial reagents in a clinical laboratory.

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RESUMO

Comparative evaluations of immune status for rubella virus are described for enzyme-linked immunosorbent assay, hemagglutination inhibition, and indirect hemagglutination. A 92.1% agreement between enzyme-linked immunosorbent assay and indirect hemagglutination assay was demonstrated for rubella immune status. Enzyme-linked immunosorbent assay and hemagglutination inhibition demonstrated a 92.6% agreement and were compared in an attempt to define the quantitative usefulness of comparisons of single sera for determining immune status. These data support the relative lack of correlation between single enzyme-linked immunosorbent assay and hemagglutination inhibition quantitative values. Enzyme immunoassay was, however, an acceptable alternative to hemagglutination inhibition for the determination of immune status to rubella virus.

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