Comparison of methods for immunocytochemical detection of rotavirus infections.

AUTOR(ES)

Graham, D Y

RESUMO

Rotavirus infections in intestinal tissues of animals or in tissue culture cells were detected by the immunocytochemical unlabeled soluble enzyme peroxidase antiperoxidase method. Comparison of the immunofluorescence and peroxidase antiperoxidase immunological staining techniques revealed that the two methods are equally sensitive for detection of rotavirus-infected cells. The peroxidase antiperoxidase technique offers the advantages of negligible nonspecific staining reactions, the use of a standard light microscope, the production of permanent slides, and the conservation of immunological reagents. The ability to detect antigens in paraffin-embedded tissues enhances the usefulness of the peroxidase antiperoxidase test for both prospective and retrospective studies.

Documentos Relacionados

• Methicillin-resistant staphylococci: detection methods and treatment of infections.
• Comparison of methods for detection of serum antibody to murine rotavirus.
• Comparison of Dacron-tipped applicator and cytobrush for detection of chlamydial infections.
• Comparison of immunofluorescence and culture methods in the diagnosis of chlamydial infections.
• Comparison of Serological Detection Methods for Diagnosis of Ehrlichia canis Infections in Dogs†