Comparison of the sequence of the gene encoding African swine fever virus attachment protein p12 from field virus isolates and viruses passaged in tissue culture.

AUTOR(ES)

Angulo, A

RESUMO

Comparison of the amino acid sequence of the African swine fever virus attachment protein p12 from different field virus isolates, deduced from the nucleotide sequence of the gene, revealed a high degree of conservation. No mutations were found after adaptation to Vero cells, and a polypeptide with similar characteristics was present in an IBRS2-adapted virus. The sequence of the 5' flanking region was conserved among the isolates, whereas sequences downstream of the gene were highly variable in length and contained direct repeats in tandem that may account for the deletions found in different isolates. Protein p12 was synthesized in swine macrophages infected with all of the viruses tested.

Documentos Relacionados

• Monoclonal antibodies of African swine fever virus: antigenic differences among field virus isolates and viruses passaged in cell culture.
• Transcriptional mapping of a late gene coding for the p12 attachment protein of African swine fever virus.
• Amino acid sequence and structural properties of protein p12, an African swine fever virus attachment protein.
• African swine fever virus attachment protein.
• Inhibition of African swine fever virus binding and infectivity by purified recombinant virus attachment protein p12.