Comparison of two methods of genetic exchange in determination of the genetic locus of the structural gene for Pseudomonas aeruginosa elastase.

AUTOR(ES)
RESUMO

A mutation, lasA1, that results in the synthesis of enzymatically inactive elastase protein was mapped by R68.45 plasmid-mediated conjugation and transposon-facilitated recombination to ca. 75 min on the Pseudomonas aeruginosa PAO chromosome. Since immunologically cross-reactive material is produced, the mutation presumably arose within the elastase structural gene.

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