Comparison of Vibrio parahaemolyticus grown in estuarine water and rich medium.
AUTOR(ES)
Pace, J
RESUMO
Cell envelope composition and selected physiological traits of Vibrio parahaemolyticus were studied in regard to the Kanagawa phenomenon and growth conditions. Cell envelopes were prepared from cells cultured in Proteose Peptone-beef extract (Difco Laboratories, Detroit, Mich.) medium or filtered estuarine water. Protein, phospholipid, and lipopolysaccharide contents varied with culture conditions. The phospholipids present in the cell envelopes were identified as phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin. Phosphatidylethanolamine decreased and phosphatidylglycerol increased in cells grown in estuarine water. Profiles of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated numerous protein species, with four to six predominant proteins ranging from 26,000 to 120,000 in molecular weight. The profile of V. parahaemolyticus cell envelope proteins was unique and might be useful in the identification of the organism. Alkaline phosphatase activity was slightly higher in Kanagawa-negative strains and was higher in cells grown in estuarine water than in cells grown in rich laboratory medium. The DNA levels in estuarine water-grown cells increased, while RNA levels and cell volume decreased. Bacteriophage sensitivity typing demonstrated a close intraspecies relationship. Results indicated that Kanagawa-positive and -negative strains were closely related, but they could be grouped separately and may have undergone starvation-related physiological changes when cultured in estuarine water.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=202973Documentos Relacionados
- Characteristics of Escherichia coli grown in bay water as compared with rich medium.
- Production of the Kanagawa hemolysin by Vibrio parahaemolyticus in a synthetic medium.
- Isolation of Vibrio parahaemolyticus and Vibrio alginolyticus from Estuarine Areas of Southeastern Alaska
- Occurrence of Vibrio parahaemolyticus in Estuarine Waters and Oysters of New Hampshire
- Medium for Isolation and Differentiation of Vibrio parahaemolyticus and Vibrio alginolyticus