Comparisons of Protocols for Decontamination of Environmental Ice Samples for Biological and Molecular Examinations

AUTOR(ES)

Rogers, S. O.

FONTE

American Society for Microbiology

RESUMO

Drilling and laboratory manipulations of glacial ice cores introduce contemporary microbes and biomolecules onto the cores. We report herein a systematic comparative study of several decontamination protocols. Only treatment with 5% sodium hypochlorite eliminated all external contaminating microbes and nucleic acids while maintaining the integrity of those within the cores.

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