Complementarity of RNA Produced by Enzymic Transcription of Native and Denatured B. subtilis DNA*

AUTOR(ES)

Cassuto, Era

RESUMO

This paper describes the preparation and some of the properties of the RNA specimens synthesized with the aid of the RNA polymerase of E. coli by transcription of the following DNA templates: (a) undenatured B. subtilis DNA (yielding N-RNA); (b) separated strand fractions L and H isolated by chromatography of the denatured DNA on methylated albumin (yielding L-RNA and H-RNA, respectively). The study of the hybridization behavior of the various RNA products showed that N-RNA, though able to form hybrids with either strand, hybridized with H-DNA to twice as great an extent as with L-DNA. The transcripts of the separated L and H fractions exhibited complete specificity with respect to complexing: L-RNA hybridized only with L-DNA, H-RNA only with H-DNA.

Documentos Relacionados

• Translational Complementarity of RNA Transcripts of Native and Denatured B. subtilis DNA*
• HYBRIDIZATION OF CHROMOSOMAL RNA TO NATIVE DNA*
• Transcription of DNA-histone complexes by yeast RNA polymerase B.
• STIMULATION OF PROTEIN SYNTHESIS IN VITRO BY DENATURED DNA*
• Seapration of B. subtilis DNA into complementary strands. II. Template functions and composition as determined by transcription with RNA polymerase.