Complementary DNAs encoding eukaryotic-type cytidine-5'-diphosphate-diacylglycerol synthases of two plant species.

AUTOR(ES)

Kopka, J

RESUMO

Cytidine diphosphate (CDP)-diacylglycerol synthase (cytidine triphosphate:phosphatidate cytihyltransferase, EC 2.7.7.41) catalyzes the formation of CDP-diacylglycerol, which is the precursor of phosphatidylinositol, phosphatidylglycerol, and cardiolipin. We report the first cloning, to our knowledge, of two plant cDNAs, StCDS1 and AtCDS1, coding for CDP-diacylglycerol synthase from potato (Solanum tuberosum) and Arabidopsis thaliana, respectively. The two proteins belong to the eukaryotic type of CDP-diacylglycerol synthase and contain eight predicted transmembrane-spanning domains. We analyzed gene expression in shoot and root tissues of potato plants and demonstrated enzyme activity by expression of N-terminally truncated, recombinant StCDS1 in Escherichia coli.

Documentos Relacionados

• Eukaryotic-type elongator tRNAMet of Trypanosoma brucei becomes formylated after import into mitochondria
• A Eukaryotic-Type Protein Kinase, SpkA, Is Required for Normal Motility of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC 6803
• Characterization of a membrane-associated cytidine diphosphate-diacylglycerol-dependent phosphatidylserine synthase in bacilli.
• Molecular cloning of complementary DNAs encoding two cationic peroxidases from cultivated peanut cells.
• Cytidine 5'-diphosphate reductase activity in phytohemagglutinin stimulated human lymphocytes.