Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences
AUTOR(ES)
Hamilton, W. D. O.
RESUMO
The nucleotide sequences of the infectious cloned DNA components of tomato golden mosaic virus (TGMV) have been determined. DNA A (2588 nucleotides) and DNA B (2508 nucleotides) have little sequence homology except for a region of ˜200 bases which is almost identical in the two molecules. Analysis of open reading frames revealed six potential coding regions for proteins of mol. wt. >10 000, four in DNA A and two in DNA B. Possible regulatory signals are identified and a model for bidirectional transcription of the two genome components is presented. Comparison of the nucleotide sequences of the DNAs of TGMV and cassava latent virus (CLV) revealed a fairly close relationship between TGMV DNA A and CLV DNA 1 and a comparatively distant relationship between TGMV DNA B and CLV DNA 2. All the potential coding regions in the TGMV DNAs had counterparts in the CLV DNAs suggesting an overall similarity in genome organisation, but six potential coding regions in the CLV DNAs had no counterparts in the TGMV DNAs. The 200-base region common to the two DNAs of each virus had little sequence homology, except for a highly conserved 33-base sequence potentially capable of forming a stable hairpin structure.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=557666Documentos Relacionados
- Nucleotide sequence of the infectious cloned DNA components of African cassava mosaic virus (Nigerian strain).
- Molecular cloning and characterisation of the two DNA components of tomato golden mosaic virus.
- S1-sensitive sites in the supercoiled double-stranded form of tomato golden mosaic virus DNA component B: identification of regions of potential alternative secondary structure and regulatory function.
- DNA sequences essential for replication of the B genome component of tomato golden mosaic virus.
- The complete nucleotide sequences of the cloned hepatitis B virus DNA; subtype adr and adw.