Composition and Size of Shope Fibroma Virus Deoxyribonucleic Acid
AUTOR(ES)
Jacquemont, B.
RESUMO
Deoxyribonucleic acid (DNA) extracted from purified virions of Shope fibroma virus (SFV) (by using DNA from Microccocus lysodeikticus as marker) had a buoyant density of 1.6996 ± 0.0003 g/ml), hence a guanine plus cytosine (G + C) content of 40.4 ± 0.3%, which is close to the G + C content of the DNA of susceptible rabbit cells (40.9 ± 0.4%) and different from that of vaccinia virus DNA (35.5 ± 0.4%). For the determination of the molecular weight of DNA, SFV and vaccinia purified virions, treated with Pronase and detergent, were cosedimented in sucrose density gradients. Results showed that SFV-DNA has a molecular weight of about 153 × 106 daltons. By electron microscopy, only one molecule corresponding to this value was observed (its length was 80.3 μm). The others had a median size of 49.8 μm ± 0.9.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=356381Documentos Relacionados
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